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Clare Coyne
Thu 12 April 2007
 59 Johnson Hall,
Washington State University,
Pullman, WA 99164-6402,
USA
Mini-biographyDr. Coyne received her B.S. degree from University of California, Davis and M.S. and Ph.D. from Oregon State University. Her post-doctoral work studying Aphanomyces root rot of pea was with Professor Fred Muehlbauer at Washington State University in 1995. She continued her work on developing markers for disease resistances in pea as a geneticist and curator with the USDA National Plant Germplasm System from 1998 to present. Dr. Coyne has authored or co-authored 36 journal articles and proceedings along with two book chapters. USDA Cool Season Food Legume Germplasm ProjectClare Coyne1; Co-authors: Allan Brown 1, Gail Timmerman-Vaughan 2, Rajeev Varshney 31. USDA-ARS, 59 Johnson Hall, WSU, Pullman, WA 99164-6402, USA, 2. New Zealand Institute for Crop and Food Research, Lincoln, New Zealand, 3. ICRISAT, Patancheru 502324, Andhra Pradesh, India coynec@wsu.eduThe USDA cool season food legume germplasm collection consists of 16,000 accessions, primarily landraces. Concentrated genotyping and phenoytping of the pea, chickpea and lentil core collections is well underway. Genotyping of the pea core collection (310 accessions) is complete for 37 mapped RAPDs and 15 microsatellites. Genotyping of the chickpea core collection (384 accessions) is complete for 20 microsatellites. Extensive field studies of yield components of the pea and chickpea core collections will be presented (Coyne, unpublished). Access to all the data and seed requests is freely available on the USDA National Plant Germplasm System web-based database at www.ars-grin.gov. Development of association mapping populations of expanded pea, chickpea and lentil core collections are also underway. Analysis of the genetic diversity of the USDA pea and chickpea core collections will be presented.Brown A.F., C.A. Watt, L. Taylor, L. Razai and CJ Coyne. 2007. Population genetic sub-structure within the USDA ARS Pisum core collection and its potential as a platform for association mapping. Theoretical and Applied Genetics (submitted).
Coyne C.J., Brown A.F., Timmerman-Vaughan G.M., McPhee K.E., Grusak, M.A. 2005. Refined USDA-ARS pea core collection based on 26 quantitative traits. Pisum Genetics 37:3-6.
Coyne CJ, Razai L, Baik B-K, Grusak M.A. 2005. Variation for pea seed protein concentration in USDA Pisum core collection. Pisum Genetics 37:7-11.
Molecular markers for resistance to fusarium wilt and root rot in pea, ascochyta blight in chickpeaClare Coyne1; Co-authors: Marie-Laure Pilet Nayel2, Niklaus Grunwald3, Kevin McPhee4, P.N. Rajesh4
1. USDA-ARS 59 Johnson Hall, WSU, Pullman, WA 99164-6402, USA; 2. INRA-APBV, 35653 Le Rheu, France; 3. USDA-ARS CGRB-OSU, Corvallis, OR 97331 4. USDA-ARS 302 Johnson Hall, WSU, Pullman, WA 99164-6434
coynec@wsu.edu
A three-year field study of fusarium root rot (causal organism Nectria haematocca; anamorph Fusarium solani) using a RIL mapping population revealed a high level of heritability for genetic resistance. Using this field data, co-dominant microsatellite markers (Loridon et al 2006) flanking three resistant QTLs to fusarium root rot in pea have been identified (Pilet-Nayel, Grunwald and Coyne, unpublished) and will be useful for marker-assisted selection (MAS) in pea. Dominant SCAR markers and co-dominant microsatellite markers for pea resistance to fusarium wilt (causal organism Fusarium oxysporum f.sp. pisi) races 1, 2, and 5 in pea (Okubara et al 2002, 2005; McClendon et al 2002; McPhee unpublished) and published markers for chickpea resistance to ascochyta blight (Ascochyta rabiei) (Rajesh et al 2003) will also be presented. A discussion of the usefulness of each marker and differential sets of accessions for each disease will be included (Chen et al 2004; Infantino et al 2006).Chen W., C. J. Coyne, T. L. Peever and F. J. Muehlbauer. 2004. Characterization of chickpea differentials for virulence assay of Ascochyta blight and identification of chickpea accessions resistant to Ascochyta rabiei. Plant Pathology 53: 759-769.
Infantino, A., M. Kharrat, L. Riccioni, C.J. Coyne, K.E. McPhee, N.J. Grünwald. 2006. Screening techniques and sources of resistance to root diseases in legumes. Euphytica 147:201-221.
Loridon K., K.E. McPhee, J. Morin, P. Dubreuil, M.L. Pilet-Nayel, G. Aubert, C. Rameau, A. Baranger, C.J. Coyne, I. Lejeune-Hénault, J. Burstin. 2005. Microsatellite marker polymorphism and mapping in pea (Pisum sativum L.). Theoretical and Applied Genetics 111:1022-1031.
Okubara P.A., Keller K.E., McClendon M.T., McPhee K.E., Inglis D.A., and Coyne C.J. 2005. Y15_999Fw, a dominant SCAR marker linked to the Fusarium wilt race 1 (Fw) resistance gene in pea. Pisum Genetics 37: 32-35.
Okubara, P.A. Inglis, D.A. Muehlbauer, F.J. Coyne, C.J. 2002. A novel RAPD marker linked to the Fusarium wilt race 5 resistance gene (Fwf) in Pisum sativum. Pisum Genetics 34:6-8.
McClendon, MT, DA Inglis, KE McPhee. and C.J. Coyne. 2002. DNA markers for Fusarium wilt race 1 resistance gene in pea. Journal of the American Society for Horticultural Science 127:602-607.
Rajesh P.N., C.J. Coyne, K. Meksem, K.D. Sharma, V. Gupta, and F.J. Muehlbauer. 2003. Construction of a HindIII bacterial artificial chromosome library and its use in identification of clones associated with disease resistance in chickpea. Theoretical and Applied Genetics 108:663-669.
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